mouse igg1 isotype control (R&D Systems)

R&D Systems is a verified supplier

R&D Systems manufactures this product

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

R&D Systems mouse igg1 isotype control
Mouse Igg1 Isotype Control, supplied by R&D Systems, used in various techniques. Bioz Stars score: 96/100, based on 842 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/isotype+mouse+igg1/Mouse+IgG1+Isotype+Control/10__1038_slash_s41598___026___72225___3-227-23-27
Average 96 stars, based on 842 article reviews

mouse igg1 isotype control - by Bioz Stars, 2026-10

96/100 stars

Images

Functional Assay:

Article Title: A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Article Snippet: .. Functional blocking: anti-CD27 blocking antibody (R&D systems, MAB382), isotype mouse IgG1 (R&D systems, MAB002). ..

Blocking Assay:

Article Title: A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Article Snippet: Recombinant Fc chimera CD70 (SinoBiological, 10780-H01H) or human IgG (R&D systems, 1-001-A) was incubated with a single-cell suspension of FL haematopoietic cells for 10 min at 4 °C in RPMI with 10% FCS. .. To block CD70–CD27 binding, cells were incubated in the presence of anti-CD27 blocking antibody (R&D systems, MAB382) or isotype mouse IgG1 (R&D systems, MAB002) for 30 min at 4 °C before binding. .. After binding, the cells were washed, fixed using 4% paraformaldehyde for 10 min at 20 °C, incubated with PE-anti-human IgG Fc (R&D systems; 1:500), FITC-anti-CD3 (1:500), APC-anti-CD19 (1:500) and PE-Cy7-anti-CD10 (1:500) for 20 min at 4 °C, and analysed using flow cytometry (FACSAria II or III) and FlowJo software.

Article Title: A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Article Snippet: Recombinant protein binding assay: Recombinant Fc chimera CD70 (SinoBiological, 10780-H01H) or human IgG (R&D systems, 1-001-A) was incubated with a single-cell suspension of FL haematopoietic cells for 10 min at 4 oC in RPMI with 10% FCS. .. To block CD70–CD27 binding, malignant B cells were incubated in the presence of anti-CD27 blocking antibody (R&D systems, MAB382) or isotype mouse IgG1 (R&D systems, MAB002) for 30 min at 4 oC before binding. .. After binding, the cells were washed, fixed by 4% PFA for 10 min at 20 oC and incubated with PE-anti-human IgG Fc (R&D systems), FITC-anti-CD3, APC-anti-CD19, and PE-Cy7-anti-CD10 for 20 min at 4 oC.

Binding Assay:

Article Title: A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Article Snippet: Recombinant protein binding assay: Recombinant Fc chimera CD70 (SinoBiological, 10780-H01H) or human IgG (R&D systems, 1-001-A) was incubated with a single-cell suspension of FL haematopoietic cells for 10 min at 4 oC in RPMI with 10% FCS. .. To block CD70–CD27 binding, malignant B cells were incubated in the presence of anti-CD27 blocking antibody (R&D systems, MAB382) or isotype mouse IgG1 (R&D systems, MAB002) for 30 min at 4 oC before binding. .. After binding, the cells were washed, fixed by 4% PFA for 10 min at 20 oC and incubated with PE-anti-human IgG Fc (R&D systems), FITC-anti-CD3, APC-anti-CD19, and PE-Cy7-anti-CD10 for 20 min at 4 oC.

Incubation:

Article Title: A single-cell atlas of non-haematopoietic cells in human lymph nodes and lymphoma reveals a landscape of stromal remodelling
Article Snippet: Recombinant protein binding assay: Recombinant Fc chimera CD70 (SinoBiological, 10780-H01H) or human IgG (R&D systems, 1-001-A) was incubated with a single-cell suspension of FL haematopoietic cells for 10 min at 4 oC in RPMI with 10% FCS. .. To block CD70–CD27 binding, malignant B cells were incubated in the presence of anti-CD27 blocking antibody (R&D systems, MAB382) or isotype mouse IgG1 (R&D systems, MAB002) for 30 min at 4 oC before binding. .. After binding, the cells were washed, fixed by 4% PFA for 10 min at 20 oC and incubated with PE-anti-human IgG Fc (R&D systems), FITC-anti-CD3, APC-anti-CD19, and PE-Cy7-anti-CD10 for 20 min at 4 oC.

FACS:

Article Title: Reduced pathological angiogenesis and tumor growth in mice lacking GPR4, a proton sensing receptor.
Article Snippet: The G protein-coupled receptor GPR4 is activated by acidic pH and recent evidence indicates that it is expressed in endothelial cells.. In agreement with these reports, we observe a high correlation of GPR4 mRNA expression with endothelial marker genes, and we confirm expression and acidic pH dependent function of GPR4 in primary human vascular endothelial cells.. GPR4-deficient mice were generated; these are viable and fertile and show no gross abnormalities.

Control:

Article Title: Antitumor activities of a novel fluorinated small molecule (A1) in CT26 colorectal cancer cells: molecular docking and in vitro studies.
Article Snippet: Chemotherapeutic treatment of colorectal cancer (CRC) has not been satisfactory until now; therefore, the discovery of more efficient medications is of great significance.. Based on available knowledge, the CXCL12/CXCR4 axis plays a significant role in tumorigenesis, and inhibition of CXCR4 chemokine receptor with AMD3100 is one of the most known therapeutic modalities in cancer therapy.. Herein, N, N’’thiocarbonylbis(N’-(3,4-dimethylphenyl)-2,2,2-trifluoroacetimidamide) (A1) was synthesized as a potent CXCR4 inhibitor.

Review